Enhancement of native fluorescence intensity of berberine by (2-hydroxypropyl)--cyclodextrin in capillary electrophoresis coupled by laser-induced fluorescence detection: Application to quality control of medicinal plants

Journal of Chromatography A


S. Uzaşçı, F.B. Erim


A sensitive method for the determination of berberine, a plant alkaloid, was developed by capillary electrophoresis coupled with laser induced fluorescence detection. With the addition of (2-hydroxypropyl)--cyclodextrin (2-HP--CD) to the separation electrolyte, the low fluorescence signal intensity of berberine in aqueous medium was enhanced considerably by 162 fold. The optimal background electrolyte was selected as 20mM acetic acid, 35mM 2-HP--CD, and 20% methanol at pH 5. The limit of detection of the method for berberine was 15.7ng/mL. The intra and interday repeatabilities of corrected peak areas as RDSs were 1.83% and 3.75%, respectively. The developed method was successfully applied for the determination of berberine contents in Chinese medicinal plants and herbal supplemental tablets.

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